Bone marrow biopsy is performed to determine whether there is any functional, structural or cellular change to the bone marrow. It is a procedure that is only necessary if there is a very high suspicion of bone marrow disease; indications include pancytopenia, persistent neutropenia, monoclonal gammopathy, non-regenerative anaemia, leukocytosis, circulating atypical lymphocytes and staging for lymphoid neoplasia.
Equipment Aseptic skin-preparation equipment Fenestrated drape Scalpel blade Sterile gloves Anticoagulant – citrate phosphate dextrose adenine (this can be taken from a blood donation bag) Bone marrow needle (Jamshidi; size dependent on patient [11 gauge is usually appropriate for dogs, 14 gauge for cats]) 10 to 20 ml syringe Microscope slides Formalin pot
Using aseptic technique, remove the stylet from the Jamshidi needle. Attach the 20 ml syringe and draw up a small volume...
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